Table 1 RT-PCR primer sequences
Gene | Primer sequences(5’-3’) | Length (bp) |
|---|---|---|
Rat GAPDH | F: ACAGCAACAGGGTGGTGGAC | 253 |
R: TTTGAGGGTGCAGCGAACTT | ||
Rat TGase-2 | F: AAGTATGATGCGTCCTTCGTG | 259 |
R: CCCCTGTCTCCTCTTTCTCTG | ||
Rat HMGB1 | F: CTTATCCATTGGTGATGTTGC | 213 |
R: TCGTCTTCCTCTTCCTTCTTT | ||
Homo GAPDH | F: TCAAGAAGGTGGTGAAGCAGG | 115 bp |
R: TCAAAGGTGGAGGAGTGGGT | ||
Homo TGase-2 | F: CACAAATCCATCAACCGTTCCC | 183 bp |
R: CATCCCTGTCTCCTCCTTCTCG | ||
Homo HMGB1 | F: TGCGAAGAAACTGGGAGAGA | 227 bp |
R: CCTCCTCCTCCTCATCCTCT | ||
Homo TLR4 | F: ACCTGTCCCTGAACCCTAT | 135 bp |
R: CTAAACCAGCCAGACCTTG | ||
Homo MYD88 | F: ACTTGGAGATCCGGCAACTG | 169 bp |
R: CATCCGGCGGCACCAATG |