Fig. 4
From: Neutrophil extracellular traps-related genes contribute to sepsis-associated acute kidney injury
Identification and enrichment analysis of NETs-related oxidative stress (OS) genes. (A) The volcano map showing the differentially expressed genes (DEGs) between SA-AKI samples and sham samples. Orange represents the significantly up-regulated gene, green represents the significantly down-regulated gene, and gray represents a gene with insignificant. (B) The heat map showing the top 10 up-regulated and top 10 down-regulated DEGs between SA-AKI and Sham samplesexpression difference. (C) Scale independence and average connectivity plots are used to select the best soft threshold for weighted gene co-expression network analysis (WGCNA). (D) The clustering tree of gene clusters in WGCNA. (E) The heat map showing the correlation between gene modules and ssGSEA scores of OS. (F) A venn diagram was used to obtain the genes in the intersection of the genes in MEbrown and MEroyalblue modules (OSG_module), DEGs between SA-AKI samples and Sham samples (Groups_Degs), and DEGs between the two subgroups of SA-AKI (Cluster_Dges). (G) String diagram of the top 20 items of GO analysis based on the genes in the intersection. (H) String diagram of the top 10 items of KEGG enrichment analysis based on the genes in the intersection