Fig. 5
From: CIAPIN1 attenuates ferroptosis via regulating PI3K/AKT pathway in LPS-induced podocytes
CIAPIN1 overexpression suppresses cellular reactive oxygen species (ROS) production and ferroptosis in LPS-induced podocyte. (A) MPC5 cells were stained with DHE, and representative images are shown (magnification 100×). (B) Quantification of DHE positive cells relative to DAPI positive cells. (C) The colorimetric method was used to determine oxidative stress indicators malondialdehyde (MDA) content in lysate of MPC5 cells. (D) The concentration of Fe2+ were measured in MPC5 cells. (E) Representative gel blots depicting levels of ferroptosis-related proteins in MPC5 cells (Normalized to GAPDH). (F, G) Quantification analysis of solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) protein bands. Data are presented as mean ± SD in triplicates, and analyzed using one-way ANOVA and Bonferroni test was used for the post-hoc test. ***P < 0.001 vs. control group; ###P < 0.001 vs. LPS + Vector group; $$$P < 0.001 vs. LPS + OE- CIAPIN1 group