Fig. 4
From: CIAPIN1 attenuates ferroptosis via regulating PI3K/AKT pathway in LPS-induced podocytes
CIAPIN1 overexpression attenuates LPS-induced podocyte injury. MPC5 cells were transfected with pCDH-CMV vector overexpressing CIAPIN1 for 48 h, and then incubated with a PI3K/Akt pathway inhibitor LY294002 (10 µM) and 50 µg/mL LPS for 24 h. (A, B) RT-qPCR was performed to measure the mRNA levels of Synaptopodin and Desmin in MPC5 cells. (C) Representative gel blots depicting levels of Synaptopodin and Desmin proteins in MPC5 cells (Normalized to GAPDH). (D, E) Quantification analysis of Synaptopodin and Desmin protein bands. Data represent the average of three independent experiments (mean ± SD). ***P < 0.001 vs. control group; ###P < 0.001 vs. LPS + Vector group; $$$P < 0.001 vs. LPS + OE- CIAPIN1 group