Fig. 1

Kidney tissue is dissociated into a single cell suspension and scRNA-seq is then performed. ScRNA-seq allows the study of rare cell types, cell state and subtype heterogeneity, disease-specific cell types, and cell-to-cell interactions via ligand-receptor analysis. Computational analyses such as pseudo-time diffusion mapping analyze the similarity and diversity of cells, consent to trace differentiation processes, clonal evolution, and cell state transitions between different cell types