Table 3 Outcomes of studies comparing the findings after different agent utilization
From: Current strategies on kidney regeneration using tissue engineering approaches: a systematic review
Outcome | Study | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Sauter (2023) [20] | Shahraki (2022) [43] | Haeublein (2022) [22] | Shahraki (2021) [23] | Keshvari (2021) [24] | Zambon (2018) [79] | He (2017) [21] | Fischer (2017) [37] | Poornejad (2016) [33] | Wang (2015) [31] | Choi (2015) [77] | Caralt (2015) [25] | Chae (2014) [29] |
Compared detergents | SDS 0.66% VS SDS 3% | TRX VS SDS | SDS 0.1% VS SDS 0.01% VS EDTA | TRX VS SDS | SLES VS SDS | SDS + DNase VS TRX + SDS | - SDS 1% - SDS 0.5% - SDS 0.25 - SDS 0.125 | SDS + DNase VS TRX + DNase VS SDC + DNase | TRX VS SDS VS Trypsin/ EDTA VS NaOH VS PAA | SDS VS TRX VS PAA VS SDC | TRX VS SDS | TRX VS TRX + SDS VS TRX + EGTA/Trypsin | SDS VS TRX |
Cell removal | SDS 3% | SDS | SDS 0.1% | SDS | SLES, SDS | SDS + DNase, TRX + SDS | All were successful | SDC + DNase, SDS + DNase | SDS NaOH | SDS | SDS | TRX + SDS | SDS |
Environment for further cell proliferation/ adhesion/ viability | SDS 0.66% | TRX | EDTA | SDS | SLES > SDS | NM | NM | SDC + DNase | PAA NaOH | NM | TRX | TRX + SDS | TRX |
GAG preservation | NM |  | NM | NM | SLES, SDS | NM | 4 h | SDS + DNase | TRX (> 65%) SDS (> 65%) EDTA/ Trypsin (42%) Min: NaOH (25%) | Min: SDS + TRX | NM | NM | NM |
GF retention | NM |  | NM | NM | NM | NM | 4 h/ SDS 0.125% | SDC + DNase | TRX (> 86%) PAA (> 53%) NaOH (> 33%) Min: SDS (16%) | NM | TRX | Min: TRX + EGTA/Trypsin Max: TRX, TRX + SDS | TRX |
Collagen preservation | NM |  | NM | SDS | SLES | NM | NM | SDS + DNase at 4 °C | TRX (> 90%) NaOH (> 90%) PAA (> 90%) EDTA/ Trypsin (36%) SDS (50%) | SDS + TRX | TRX | TRX + SDS | NM |
Lower DNA content | SDS 3% | SDS | SDS 0.1% | SDS | SLES (0) < SDS (1.2) | SDS + DNase, TRX + SDS | All were successful | SDC + DNase, SDS + DNase | Min: NaOH (2%) Max: TRX | SDS (22.1) TRX (193.2) PAA (111.6) SDC (84.2) | SDS | TRX + SDS (3.08%) TRX (89.14%) TRX + EGTA/Trypsin (22.47%) | SDS |
Protein retention | NM | Â | NM | NM | SDS | NM | NM | NM | NM | Min: SDS Max: TRX | TRX | NM | TRX |
Cytokine retention | NM |  | NM | NM | NM | NM | NM | SDC + DNase | NM | NM | NM | NM | NM |
More cellular debris | SDS 0.66% |  | NM | NM | NM | NM | NM | NM | NM | NM | NM | TRX + EGTA/Trypsin | NM |
Optimal biomechanical properties | NM | Â | NM | NM | NM | NM | NM | NM | NM | NM | SDS | NM | SDS |
Microstructure scoring (structure) | NM |  | NM | NM | SLES | TRX + SDS | NM | SDC + DNase at 4 °C | NM | NM | TRX | TRX, TRX + SDS | NM |
ECM preservation | NM |  | SDS 0.01%, EDTA | SDS | SLES | TRX + SDS | NM | SDS + DNase | NM | NM | TRX | TRX, TRX + SDS | NM |
Angiogenesis | SDS 0.66% |  | NM | NM | SLES, SDS | TRX + SDS | NM | NM | NM | NM | NM | NM | NM |
Vascular patency | NM |  | NM | NM | NM | TRX + SDS | NM | NM | NM | NM | NM | NM | NM |